What questions should you ask when buying a Spectrophotometer? (Part-02)
We can pick the right instrument by considering the following specifications,
Software and Hard requirements:
Microprocessor based UV/VIS Spectrophotometer display operation on 220-250 V/50Hz.
Complete control through PC with Software.
High end True Double Beam optics with Holographic Grating in Czerny-Turner mounting.
High intensity Tungsten Halogen and Deuterium lamp with automatic changeover.
Built in validation program diagnostic and security functions.
Operational modes as standard Photometric, quantitative, Spectrum scan, Kinetics, DNA and Protein., GLP/GMP protocol.
Large sample compartment with built in 1 Reference and 1 Sample holder
Compatible with wide range of accessories such as Peltier Cool Accessories (Minimum = 50C or less and Maximum = 750C or above): 1 number
10 mm Quartz Cell: 4 numbers
Operation Qualification requirements
Wavelength range : 190 – 900 nm
Spectral Bandwidth : Continuous changeable slit from 0.1 – 5.0 nm
Stray light: 0.01% T (220 nm NaI, 340nm NaNO2).
Wavelength Accuracy: ± 0.3nm for entire range (automatic wavelength correction).
Resolution: 0.1 nm
Wavelength Repeatability : < 0.1 nm
Photometric range : -4.0A – 4.0A
Photometric Accuracy : ±0.002Abs (0-0.5Abs);
±0.004Abs (0.5-1.0Abs);
±0.3% T (0-100%T)
Photometric Repeatability : ±0.001 Abs ( 0-0.5 Abs);
± 0.002Abs (0.5–1.0Abs)
Noise : <0.00005A
Drift : 0.0004A/1h
Baseline Flatness : ±0.0005A/h
Communication Interface : USB/RS 232.
Physical conditions of spectrophotometers:
Weight: 40-60 Kg
Dimensions (Width, Depth, Height): 500-570 mm, 550-600 mm, 250-300 mm
Operating temperature: 15-35 °C
A warranty of 12 months from the date of installation should be given.
Two copies of the manuals (operation, trouble shooting& electrical manual in English versions) should be provided.
The supplier should install the system in our laboratory and demonstrate all the technical specifications.
